A Solid Phase, Sandwich-Type Radioimmunoassay forAntithyroglobulin: Elimination of False Positive Resultsand Semiquantitative Measurement of Antithyroglobulinin the Presence of Elevated Thyroglobulin*
- 1 October 1979
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 49 (4) , 565-571
- https://doi.org/10.1210/jcem-49-4-565
Abstract
Thyroid disorders can be associated with elevatedconcentrations of serum antithyroglobulin antibodies(anti-Tg) and/or thyroglobulin (Tg), but none of the availableanti-Tg assays deals with anti-Tg measurements in the presenceof abnormally high Tg levels. The competitive binding radioassay produces falsely elevatedvalues for anti-Tg if serum Tg is elevated and either falselyelevated or depressed values if both Tg and anti-Tg are abnormallyhigh. The falsely elevated anti-Tg values can be identifiedby measuring the formation of Tg-[I25I]anti-Tg complexes in thesupernatant of the anti-Tg assay (supernatant assay). For screening purposes, we modified the original anti-Tg RIAinto a solid phase, sandwich-type RIA. Anti-Tg in serum orstandard is first bound to plastic cups coated with Tg and thenquantitated by binding of [125I]Tg. This assay has a detectionlimit of 2 U%ml serum, intra- and interassay coefficients ofvariation of 9-15%, and a normal range of J ClinEndocrinol Metab49: 565, 1979)Keywords
This publication has 1 reference indexed in Scilit:
- Plasma Thyroglobulin in Detecting Thyroid Carcinoma after Childhood Head and Neck IrradiationAnnals of Internal Medicine, 1977