Study of Tropomyosin Labelled with a Fluorescent Probe by Pulse Fluorimetry in Polarized Light
Open Access
- 1 August 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 88 (2) , 421-424
- https://doi.org/10.1111/j.1432-1033.1978.tb12464.x
Abstract
Tropomyosin has been labelled with a fluorescent probe N-iodoacetyl-N′-(5-sulfo-1-naphthyl)-ethylenediamine which is presumed to bind preferentially to the unique reactive cysteine residue of the α chain. Anisotropy decay measurements show that tropomyosin monomer and polymer are flexible molecules. This flexibility decreases when troponin interacts with tropomyosin, and is partially restored by a micromolar concentration of Ca2+.This publication has 11 references indexed in Scilit:
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