Stimulation of Prolactin Secretion by Perphenazine in Pituitary-Hypothalamus Organ Culture.

Abstract
Summary Pituitary explants on medium “199′”released an average of 0.05 IU prolactin daily. Pituitary-hypothalamus co-cultures released only an average of 0.025 IU/d. Other brain parts in co-culture failed to depress prolactin release, nor did hypothalami of perphenazine-primed rats or hypothalami to which perphenazine was added in vitro. The method described is a valuable tool for appraisal of prolactin releasing or inhibiting agents.