Chaperonins dependent increase of Cu,Zn Superoxide dismutase production in Escherichia coli
- 3 May 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 322 (1) , 6-9
- https://doi.org/10.1016/0014-5793(93)81099-l
Abstract
Over‐expression of the chaperonins GroEL and GroES significantly suppressed the temperature‐dependent pattern of expression of Cu,Zn Superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal‐deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn Superoxide dismutase in vitro, confirming that GroEL can interact with β‐stranded proteins.Keywords
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