Chromosomes of 14 Hematopoietic Cell Lines Derived From Peripheral Blood of Persons With and Without Chromosome Anomalies2

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Abstract
The chromosome constitutions of 14 hematopoietic cell lines derived from peripheral blood of 11 normal persons and 3 persons with chromosome anomalies (46 + 1 minute, XYY, and XXY) were studied. Of the 11 normal lines, 8 had normal diploid karyotypes when first examined at culture ages varying from 3–23 months. Of the remaining 3 normal lines, 2 had hyperdiploid modes of 47, and 1 had a mixed mode of diploid and pseudodiploid when first examined, all at culture ages greater than 15 months. Four of the originally diploid lines changed to pseudodiploid modes when they were reexamined 20–33 months after initiation. The line with a mixed diploid and pseudodiploid mode had shifted to a hypodiploid mode of 45 at later examinations. This line and its 2 sublines all exhibited high incidences of cells with chromosome attenuation at the centromeric regions of #1, #16, and one chromosome of group G The 3 lines derived from persons with chromosome anomalies have maintained the donors' karyotypes for 10–12 months in vitro. No C chromosome with a subterminal secondary constriction, as has been reported to occur in some Burkitt cell lines, was observed in the lines studied, whether positive or negative for herpes-type virus. It is apparent that human hematopoietic cell lines can maintain the donors' chromosome constitutions as well as rapid growth rates for long periods and hundreds of cell generations in vitro. When chromosome changes finally do occur, they are minor and fairly consistent within each line.

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