DEFECTIVE TUMORICIDAL CAPACITY OF MACROPHAGES FROM A-J MICE .1. CHARACTERIZATION OF THE MACROPHAGE CYTOTOXIC DEFECT AFTER INVIVO AND INVITRO ACTIVATION STIMULI

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (4) , 1587-1591
Abstract
Macrophages from A/J mice fail to develop tumoricidal activity after any of several in vivo or in vitro treatments that activate cells from C3H/HeN mice. Peritoneal macrophages from A/J mice treated i.p. with viable Mycobacterium bovis, strain BCG, killed Corynebacterium parvum [Propionibacterium acnes] or pyran copolymer fail to develop in vitro tumoricidal activity; varying the numbers of macrophages from treated mice added to target cells, the dose and time of treatment or the treatment schedule of these in vivo activation stimuli did not evoke cytotoxic activity. Cytotoxic activity by macrophages from A/J mice was not observed with any of 4 target cell lines derived from 3 different mouse strains. In vitro treatment of peritoneal exudate macrophages from A/J mice with lymphokine-rich supernatants, bacterial [Escherichia coli] endotoxins, or T [thymus-derived] cell mitogens was ineffective; varying the numbers of treated macrophages added to target cells, the dose of in vitro inactivation stimuli or the time of treatment did not evoke cytotoxic activity. A/J mice exhibit a profound defect in macrophage tumoricidal capacity to in vivo and in vitro activation stimuli over a wide range of experimental conditions.

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