Effect of verapamil on the calcium and magnesium transports of rat kidney cortex mitochondria.

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Abstract
The effect of verapamil on Ca2+ and Mg2+ accumulation was studied in isolated rat kidney cortex mitochondria. For the 50% inhibition of Ca2+ accumulation, 2 .times. 10-4 M verapamil concentration was required in the presence of ATP (2 mM) and phosphate (5 mM). Omission of phosphate from the medium increased the inhibitory effect of verapamil on Ca2+ accumulation. Verapamil had no effect on Ca2+ accumulation in the presence of both ATP and succinate (7.8 mM), but further addition of phosphate caused a significant inhibition of Ca2+ accumulation by verapamil. Mg2+ accumulation of mitochondria was similarly depressed by verapamil. The same tendency was found as for the modification of verapamil effect by acetate in mitochondrial Ca2+ and Mg2+ accumulation. Succinate oxidation of mitochondria was not affected by verapamil in the absence of phosphate, but was inhibited by verapamil in the presence of phosphate. The depression of Ca2+ and Mg2+ transport of mitochondria by verapamil is apparently modulated by permeant anions.

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