Partial Purification and Reconstitution of the α-Ketoglutarate Carrier from Corn (Zea mays L.) Mitochondria
- 1 August 1991
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 96 (4) , 1003-1007
- https://doi.org/10.1104/pp.96.4.1003
Abstract
The alpha-ketoglutarate carrier from corn shoot mitochondria (Zea mays L., B 73) was solubilized in Triton X-114 and partially purified by chromatography on hydroxyapatite and celite in the presence of cardiolipin. On SDS-gel electrophoresis, the hydroxyapatite/celite eluate showed various protein bands between 12 and 70 kilodaltons. When reconstituted into liposomes, the alpha-ketoglutarate transport protein catalyzed a phthalonate-sensitive alpha-ketoglutarate/alpha-ketoglutarate exchange. The protein was purified 60-fold with a recovery of 88% with respect to the mitochondrial extract. The protein yield was 0.6%. The properties of the reconstituted carrier, i.e. requirement for a counter-anion, substrate specificity, and inhibitor sensitivity, were similar to those of the alpha-ketoglutarate transport system as characterized in plant and animal mitochondria.Keywords
This publication has 14 references indexed in Scilit:
- Identification and purification of the carnitine carrier from rat liver mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
- Identification and purification of the tricarboxylate carrier from rat liver mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1989
- Isolation and Partial Characterization of the Glutamate/Aspartate Transporter from Pea Leaf Mitochondria Using a Specific Monoclonal AntibodyPlant Physiology, 1989
- Molecular aspects of isolated and reconstituted carrier proteins from animal mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1989
- Purification and reconstitution of two anion carriers from rat liver mitochondria: The dicarboxylate and the 2-oxoglutarate carrierBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1988
- The extraction and reconstitution of the α-cyanocinnamate-sensitive pyruvate transporter from castor bean mitochondriaBiochemical and Biophysical Research Communications, 1986
- Purification of reconstitutively active α-oxoglutarate carrier from pig heart mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Glycine Metabolism and Oxalacetate Transport by Pea Leaf MitochondriaPlant Physiology, 1981
- Mechanism of the Exchanges Catalysed by the Oxoglutarate Translocator of Rat‐Heart MitochondriaEuropean Journal of Biochemistry, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970