Rapid quantitative assessment ofTheileria infection in ticks

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Abstract
Summary A simplified method for methyl green pyronin staining is described forTheileria parva andT. annulata in whole salivary glands ofRhipicephalus appendiculatus andHyalomma anatolicum subspecies respectively. The stain gives results comparable with Feulgen staining and can be used after the ticks have been in cold storage for 3 days. There is considerable variability in the rate and intensity of infection of these ticks with theilerial parasites and it is concluded that the method permits large samples (60 ticks per person per day) to be examined to overcome this variability when assessing infection quantitatively.