Application of a backscattered electron image to immunocytochemistry in freeze-cracked tissues.
- 1 January 1984
- journal article
- research article
- Published by International Society of Histology & Cytology in Archivum histologicum japonicum
- Vol. 47 (5) , 553-557
- https://doi.org/10.1679/aohc.47.553
Abstract
A backscattered electron (BSE) image was applied to the observation of freeze-cracked tissue blocks stained with the indirect immunoperoxidase method. On the cracked surface of the pancreas, the BSE image clearly revealed the immunostained B cell granules as bright spots. A pair of secondary electron and BSE images on a fractured surface are useful for the three-dimensional localization of antigens in cells.Keywords
This publication has 0 references indexed in Scilit: