Diphosphopyridine nucleotide pyrophosphorylase in the nuclei isolated from poisoned and regenerating rat liver

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Abstract
The procedure of Hogeboom et al. (1952) for the isolation of liver nuclei has been modified. Some properties of the iso-lated nuclei have been examined. They do not catalyse a [p32]phosphate-adenosine triphosphate exchange reaction but they do contain more adeno-sine triphosphatase activity than can be explained by contamination with mitochondria. Little change was found in the specific activity of diphos-phopyridine nucleotide pyrophosphorylase of nuclei isolated from livers of rats poisoned with the hepatotoxic agents beryllium, CCl4, dimethyl-nitrosamine and ethionine. After partial hepatectomy, the specific activity of diphosphopyridine nucleotide pyrophosphorylase of nuclei isolated from the regenerating liver falls continuously for 3 days to approximately half of its control value.