Method for Characterizing Sulfated Glycoproteins in a Glycosylation Site-Specific Fashion, Using Ion Pairing and Tandem Mass Spectrometry
- 21 January 2006
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (4) , 1181-1190
- https://doi.org/10.1021/ac051554t
Abstract
Structural analysis of sulfated glycans is essential in understanding their biological significance. Here, we present a new approach to characterize sulfated glycans present on glycoproteins. The analysis is performed on glycopeptides, so information about the sulfated species is obtained in a glycosylation site-specific manner. This method employs an ion-pairing reagent to stabilize the SO3 group of the glycopeptide, allowing useful information to be obtained during MS/MS experiments. The amount of structural information obtained from (+)ESI-MS/MS of the ion-pair complexes for sulfated glycopeptides of equine thyroid stimulating hormone is compared with information obtained by (−)ESI-MS/MS of the underivatized, sulfated glycopeptides. The results indicate that this new method provides detailed insights into the sequence, branching, and type of N-glycans present, compared to analysis via (−)ESI-MS/MS.Keywords
This publication has 21 references indexed in Scilit:
- Glycoprotein profiling by electrospray mass spectrometryJournal of the American Society for Mass Spectrometry, 2004
- Mass spectrometry of oligosaccharidesMass Spectrometry Reviews, 2004
- TNF- increases the carbohydrate sulfation of CD44: induction of 6-sulfo N-acetyl lactosamine on N- and O-linked glycansGlycobiology, 2002
- Analysis of Heparan Sulfate Oligosaccharides with Ion Pair-Reverse Phase Capillary High Performance Liquid Chromatography-Microelectrospray Ionization Time-of-Flight Mass SpectrometryJournal of the American Chemical Society, 2002
- Occurrence and Structural Analysis of Highly Sulfated Multiantennary N‐linked Glycan Chains Derived from a Fertilization‐Associated Carbohydrate‐Rich Glycoprotein in Unfertilized Eggs of Tribolodon hakonensisEuropean Journal of Biochemistry, 1996
- Mass spectrometric molecular-weight determination of highly acidic compounds of biological significance via their complexes with basic polypeptides.Proceedings of the National Academy of Sciences, 1994
- Structures of sulfated oligosaccharides in human trachea mucin glycoproteinsMolecular and Cellular Biochemistry, 1993
- The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociationJournal of the American Society for Mass Spectrometry, 1992
- A hepatic reticuloendothelial cell receptor specific for SO4-4GalNAcβ1, 4GlcNAcβ1,2Manα that mediates rapid clearance of lutropinCell, 1991
- Metabolic labeling of lutropin with [35S]sulfate.Proceedings of the National Academy of Sciences, 1981