The photocycle of bacteriorhodopsin immobilized in poly(vinyl alcohol) film
- 8 July 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 285 (1) , 66-70
- https://doi.org/10.1016/0014-5793(91)80726-j
Abstract
The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA—H2O mixture. The bacteriorhodopsin photocycle in the PVA—H2O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation to the M412‐O640 and O640‐bRa11‐trans steps. In the PVA film the O640 intermediate disappeared and a negative photoelectric signal appeared.Keywords
This publication has 13 references indexed in Scilit:
- Light- and dark-adaptation of bacteriorhodopsin measured by a photoelectric methodBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1988
- Time-resolved photoelectric and absorption signals from oriented purple membranes immobilized in gelJournal of Biochemical and Biophysical Methods, 1985
- Arrhenius parameters of the bacteriorhodopsin photocycle in dried oriented samplesBiophysical Journal, 1985
- Displacement current on purple membrane fragments oriented in a suspensionBiophysical Chemistry, 1983
- Photoelectric signals from dried oriented purple membranes of Halobacterium halobiumBiophysical Journal, 1983
- Hydrogen bonded chain mechanisms for proton conduction and proton pumpingThe Journal of Membrane Biology, 1983
- Fast components of the electric response signal of bacteriorhodopsin proteinActa Physica Academiae Scientiarum Hungaricae, 1982
- THE EFFECT OF VISCOSITY ON THE PHOTOCYCLE OF BACTERIORHODOPSINPhotochemistry and Photobiology, 1981
- Photocurrent kinetics of purple-membrane sheets bound to planar bilayer membranesThe Journal of Membrane Biology, 1981
- [69] Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membranePublished by Elsevier ,1974