Two nonallelic tRNAiMet genes are located in the p23 leads to q12 region of human chromosome 6.

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Abstract
Two nonallelic human .**GRAPHIC**. [methionine tRNA] genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each .**GRAPHIC**. gene. These unique sequence probes thus allowed each .**GRAPHIC**. gene to be analyzed individually in cell hybrids. Both .**GRAPHIC**. genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into 5 segments, both .**GRAPHIC**. genes were assigned to the p23 .fwdarw. q12 region. Other .**GRAPHIC**. genes may be syntenic with the 2 described in this study. The utility of using unique flanking sequences to identify members of a multigene family was illustrated.