Incorporation and Asymmetric Orientation of Glycophorin in Reconstituted Protein‐Containing Vesicles
Open Access
- 1 May 1978
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 86 (2) , 539-546
- https://doi.org/10.1111/j.1432-1033.1978.tb12337.x
Abstract
Glycophorin can be incorporated in lipid vesicles without the use of detergents [MacDonald, R. J. and MacDonald, R. C. (1975) J. Biol. Chem. 250, 9206–9214]. In this paper it has been shown that the protein spans the lipid bilayer in these vesicles. In addition it has been shown that the protein is orientated in an asymmetric way with 75–80% of its sugar residues directed to the outside of the vesicles. Freeze-fracturing electron microscopy suggests that the protein is present in a slightly aggregated form. Sonication of these recombinants leads to the formation of small glycophorin-containing vesicles, in which almost all sugar residues are directed to the outside.Keywords
This publication has 35 references indexed in Scilit:
- The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatographyBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- Membrane AsymmetryScience, 1977
- Evidence for the preferential interaction of glycophorin with negatively charged phospholipidsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- A new, simple procedure for the isolation of sialoglycoproteins from human erythrocyte membranes of ABO blood group activitiesFEBS Letters, 1976
- Lectin-mediated attachment of glycoprotein-bearing liposomes to cellsNature, 1976
- Lectin-receptor interactions in liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Fractionation of the major sialoglycopeptides of the human red blood cell membraneBiochemical and Biophysical Research Communications, 1975
- Subunit structure of the major human erythrocyte glycoprotein: Depolymerization by heating ghosts with sodium dodecyl sulfateBiochemical and Biophysical Research Communications, 1973
- Preparative isolation of phosphatidyl serine from brainBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- A rapid and sensitive sub-micro phosphorus determinationAnalytica Chimica Acta, 1961