Human pancreatic phospholipase A2stimulates the growth of human pancreatic cancer cell line

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Abstract
Phospholipase A2 (PLA2) from human pancreas, designated hPLA2‐I, functions as a digestive enzyme. Interestingly, the present study demonstrated that the mature form of hPLA2‐I stimulated the growth of a human pancreatic cancer cell line MIAPaCa‐2, whereas the pro‐form was ineffective. PLA2s from Laticauda semifasciata fraction I, Crotalus adamanteus venom, Streptomyces violaceoruber and bee venom, showed no proliferative effect to the growth of MIAPaCa‐2. The Scatchard plot analysis revealed that the MIAPaCa‐2 cell had a specific binding site for the mature hPLA2‐I. The equilibrium binding constant (K d) and the maximum binding capacity (B max) were 2.6 nM and 0.4 fmol/106 cells, respectively. These results suggest that the mature hPLA2‐I, but not the pro‐form, may function as a growth factor of pancreas carcinoma via the specific binding site.