Isolation and characterisation of an isoproturon-mineralisingMethylopilasp. TES from French agricultural soil
Open Access
- 1 October 2004
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 239 (1) , 103-110
- https://doi.org/10.1016/j.femsle.2004.08.017
Abstract
Using enrichment culture three isoproturon (IPU) mineralising bacterial isolates were isolated from a French agricultural soil mineralising up to 50% of the initially added 14C-ring labelled IPU within only eight days. These isolates showed similar metabolic (BIOLOG GN) and amplified rDNA restriction (ARDRA) profiles. Partial 16S rDNA sequencing revealed that they were identical and identified as Methylopila sp TES. This strain harbours a large plasmid (220 kb) putatively bearing essential IPU-degrading genes as demonstrated by a curing experiment. Methylopila sp. TES transformed IPU and its known metabolites to CO2 and biomass but did not degrade chlorotoluron, monolinuron, diuron and linuron.Keywords
This publication has 37 references indexed in Scilit:
- Atrazine catabolism by a combined bacterial association (KRA30) under carbon- and nitrogen-limitations in a retentostatJournal of Applied Microbiology, 2003
- Growth in Coculture Stimulates Metabolism of the Phenylurea Herbicide Isoproturon by Sphingomonas sp. Strain SRS2Applied and Environmental Microbiology, 2002
- Isolation from Agricultural Soil and Characterization of a Sphingomonas sp. Able To Mineralize the Phenylurea Herbicide IsoproturonApplied and Environmental Microbiology, 2001
- Degradation of Substituted Phenylurea Herbicides by Arthrobacter globiformis Strain D47 and Characterization of a Plasmid-Associated Hydrolase Gene, puhAApplied and Environmental Microbiology, 2001
- Mecoprop, Isoproturon, and Atrazine in and above a Sandy Aquifer: Vertical Distribution of Mineralization PotentialEnvironmental Science & Technology, 2000
- Methylopila capsulata gen. nov., sp. nov., a novel non-pigmented aerobic facultatively methylotrophic bacteriumInternational Journal of Systematic and Evolutionary Microbiology, 1998
- Metabolism of Isoproturon in Soils Originating from Different Agricultural Management Systems and in Cultures of Isolated Soil BacteriaInternational Journal of Environmental Analytical Chemistry, 1996
- Effects of the phenylurea herbicide isoproturon on periphytic diatom communities in freshwater indoor microcosmsEnvironmental Pollution, 1996
- A method to assess the toxicity of pollutants on anaerobic microbial degradation activity in sedimentsEnvironmental Toxicology & Water Quality, 1994
- Changes in theRhizobium melilotiGenome and the Ability to Detect Supercoiled Plasmids During Bacteroid DevelopmentMolecular Plant-Microbe Interactions®, 1990