Suitability of some enrichment broths and diluents for enumerating cold- and heat-stressed Vibrio parahaemolyticus

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Abstract
V. parahaemolyticus cells were injured by chilling and heating, and their recovery was tested in glucose-salt-Teepol broth (GSTB), tryptic soy broth containing 7% NaCl(TSBS), Horie-arabinose-ethyl violet broth (HAEB), and water blue-alizarin yellow broth (WBAY). Exponential phase cells were more sensitive to cold shock than were stationary phase cells. Exposure of chill-injured V. parahaemolyticus to GSTB and TSBS resulted in 70-80% death; about 70% lethality was noted for heat-injured cells inoculated into TSBS. Neither HAEB nor WBAY enrichment media were lethal to stressed cells, although rates of growth were retarded. The 3% of NaCl in 0.1 M potassium phosphate (pH 7.0) diluent was most suitable for protecting against inactivation of cold- and heat-injured cells. [This study relates to detecting bacteria in foods.].

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