ON MICROFLUORIMETRIC SEPARATORY DETERMINATION OF ESTROGENS IN THE BLOOD AND URINE

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Abstract
Bates and Cohen (1950 a, b) and jayle and Crepy (1952) have already reported on the microfluorometry of estrogens a few years ago. Fluorescence spectra were measured comparatively precisely and reported by Slaumwhite et al. (1953) and by Aitken and Preedy (1953). As for the limit of such measurements, Braunsberg et al. (1954) reported that a very minute amount could be detected.The present authors also reported on the fluorescence spectra of natural estrogens (Nakao and Aizawa, 1955) and described the method whereby the minimum amount could be determined.Numerous reports have appeared regarding the method of isolation of natural estrogens, such as the chromatography (Stimmel, 1944) and counter-current distribution (Engel et al., 1950) and more especially the microchromatography by Braunsberg et al. (1954).The present paper describes the separatory determination of estrogens in the blood and urine established by the writers.

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