The Characteristics of the Cell-free Translation of mRNA from Cells Infected with the Herpes Virus Pseudorabies Virus
- 1 August 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 49 (2) , 323-332
- https://doi.org/10.1099/0022-1317-49-2-323
Abstract
The translation in vitro of mRNA from pseudorabies virus infected cells was studied using systems derived from wheat germ and from rabbit reticulocyte. The mRNA was shown by molecular hybridization to contain sequences complementary to virus DNA. Products of in vitro translation co-migrating with virus proteins on polyacrylamide gel electropherograms were detected, and the major capsid protein (MW 150,000) was present among the products by immune precipitation. Optimum conditions for the stimulation of amino acid incorporation in vitro were similar for mRNA from infected and mock-infected [human cervical carcinoma HeLa] cells.This publication has 7 references indexed in Scilit:
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