The hemimethylated replication origin of Escherichia coli can be initiated in vitro
Open Access
- 1 July 1991
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (14) , 4537-4539
- https://doi.org/10.1128/jb.173.14.4537-4539.1991
Abstract
Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.Keywords
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