In Vitro Demonstration of Complement-Dependent Cytotoxic Antibodies to Moloney Sarcoma Cells

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Abstract
An in vitro microcytotoxicity assay was used to demonstrate complement-dependent cytotoxic antibodies to Moloney sarcoma virus-induced cells throughout the stages of tumor progression and regression in BALB/c mice. Complement-dependent cytotoxic antibodies were present in the sera of mice whose tumors were in the late stages of tumor growth, and most consistently in the sera of mice whose tumors had completely regressed (regressors). Regressor sera were strongly cytotoxic for at least 125 days after the onset of tumor regression. Preliminary evidence indicates that the complement-dependent cytotoxic antibody in regressor sera is a 7S immunoglobulin.