Abstract
13C-NMR evidence shows that verazine [(25S)-iminocholesta-5,22(N)-diene-3β-ol] isolated from the Chinese plant Veratrum maackii (Melanthiaceae) consists of a 20R/20S mixture of epimers. The determination of the configurations at the chiral epimeric center and of the conformation of the 17β-side chain is based on the total assignment of the 13C- and 1H-NMR resonances. This goal was achieved by the extensive use of homo- and heteronuclear shift correlation and two-dimensional NOE spectroscopy methods at high magnetic field.

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