Epitopes associated with major histocompatibility complex (MHC) restriction site of T cells IV. I-J epitopes on MHC-restricted cloned T cells

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Abstract
We studied the expression of an I‐Jk epitope on class II‐restricted cloned L3T4+ T cells established from H‐2k, H‐2b, F1 and semiallogeneic radiation bone marrow chimeras by the inhibition of antigen‐induced T cell proliferation and in vitro secondary antibody response, and by the direct immunofluorescence with a monoclonal anti‐I‐Jk. Both I‐Ak‐ and I‐Ek‐restricted T cells were shown to carry the identical I‐Jk epitope regardless of their genotypic origins, antigen specificity, and helper or suppressor function. None of the I‐Ab‐restricted clones derived from similar animals showed the I‐Jk epitope. This isomorphism, regardless of the restriction specificity for I‐Ak or I‐Ek, contradicts the idea that I‐J is an idiotypic determinant on class II‐restricted T cell antigen receptor (TcR). In fact, the I‐Jk epitope was not comodulated with TcR/T3 complex when incubated with an anti‐T3 antibody, indicating that I‐J is a new isomorphic receptor for self different from TcR α/β heterodimers.

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