Fluorescent Cell-Counting Neutralization Test for Psittacosis

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Abstract
Hahon, Nicholas (Fort Detrick, Frederick, Md.), and Kenneth O. Cooke . Fluorescent cell-counting neutralization test for psittacosis. J. Bacteriol. 89: 1465–1471. 1965.—A sensitive, precise, and specific serological procedure, the fluorescent cell-counting neutralization test, was developed to detect and to measure quantitatively psittacosis serum-neutralizing antibodies within 24 hr. The test is based on the reduction of fluorescent cells in McCoy cell monolayers resulting from the neutralization of infective agent particles by specific antiserum. Small but significant rises in neutralizing titers were measured in serum specimens from monkeys previously exposed to the psittacosis agent and from humans with diagnoses of subclinical or established psittacosis infections.