Genetic recombination of bacteriophage T7 in vivo studied by use of a simple physical assay

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Abstract
A new physical method was developed to assay genetic recombination of phage T7 in vivo. The assay utilized T7 mutants that carry unique restriction sites and was based in the detection of a new restriction fragment generated by recombination. This assay was used to reexamine the genetic requirements for recombination of T7 DNA. Results agreed with previous findings that recombination required the products of genes 3 (endonuclease), 4 (primase), 5 (DNA polymerase) and 6 (exonuclease). Recombination was independent of DNA ligase and of DNA packaging and maturation functions.