DNase I hypersensitive sites in Drosophila chromatin occur at the 5' ends of regions of transcription.
- 1 January 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (1) , 143-146
- https://doi.org/10.1073/pnas.78.1.143
Abstract
By using a map of the unique region of DNA encoding the fur small heat-shock proteins of Drosophila melanogaster (hsp 22, hsp 23, hsp 26, and hsp 28), and a simple mapping technique, the positions of the DNase I hypersensitive sites of chromatin in the vicinity of these genes have now been determined. The major chromatin-specific sites occur at the 5' ends of each of the four heat-shock protein genes in embryo nuclei. These genes are not active in the nuclei analyzed but can be quickly induced in these cells by the heat-shock stimulus. The chromatin structure indicated by DNase I hypersensitivity may be a necessary factor in the general mechanism of gene activation.This publication has 16 references indexed in Scilit:
- The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase INature, 1980
- Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNACell, 1980
- Specific interaction of a purified transcription factor with an internal control region of 5S RNA genesCell, 1980
- Staphylococcal nuclease makes a single non-random cut in the simian virus 40 viral minichromosomeJournal of Molecular Biology, 1979
- The induction of gene activity in drosophila by heat shockCell, 1979
- The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequenceCell, 1979
- The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activityCell, 1979
- A stretch of “late” SV40 viral DNA about 400 bp long which includes the origin of replication is specifically exposed in SV40 minichromosomesCell, 1979
- Sites in simian virus 40 chromatin which are preferentially cleaved by endonucleasesCell, 1978
- Genes transcribed at diverse rates have a similar conformation in chromatinProceedings of the National Academy of Sciences, 1977