Isolation of a Rhodobacter capsulatus mutant that lacks c-type cytochromes and excretes porphyrins
- 1 March 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 172 (3) , 1321-1326
- https://doi.org/10.1128/jb.172.3.1321-1326.1990
Abstract
A Rhodobacter capsulatus mutant lacking cytochrome oxidase activity was isolated by Tn5 mutagenesis. Difference spectroscopy of crude extracts and extracted c-type cytochromes demonstrated that this mutant completely lacked all c-type cytochromes. The strain did, however, synthesize normal amounts of b-type cytochromes and nonheme iron. This mutant also excreted large amounts of coproporphyrin and protoporphyrin and synthesized reduced amounts of bacteriochlorophyll, suggesting a link between the synthesis of c-type cytochromes and the expression of the tetrapyrrole biosynthetic pathway. ImagesThis publication has 45 references indexed in Scilit:
- Isolation of the structural genes for the Rieske FeS protein, cytochrome b and cytochrome c1 all components of the ubiquinol: Cytochrome c2 oxidoreductase complex of Rhodopseudomonas capsulataJournal of Molecular Biology, 1987
- Rhodobacter capsulatus MT113: A single mutation results in the absence of c-type cytochromes and in the absence of the cytochrome bc1 complexBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- Soluble cytochrome composition of the purple phototrophic bacterium, Rhodopseudomonas sphaeroides ATCC 17023Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Redox chain and energy transduction in chromatophores from Rhodopseudomonas capsulata cells grown anaerobically in the dark on glucose and dimethyl sulfoxideBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1981
- Isolation and characterization of cytochrome c2‐deficient mutant of rhodopseudomonas capsulataFEBS Letters, 1980
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- SPECTROSCOPIC ANALYSIS OF BACTERIOCHLOROPHYLLS IN VITRO AND IN VIVO*Photochemistry and Photobiology, 1966
- Kinetic studies of pigment synthesis by non‐sulfur purple bacteriaJournal of Cellular and Comparative Physiology, 1957