Purification and partial characterization of a lysine-specific protease ofPorphyromonas gingivalis
- 1 October 1993
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 113 (2) , 133-137
- https://doi.org/10.1111/j.1574-6968.1993.tb06503.x
Abstract
A lysine-specific protease hydrolysing peptide bonds at the carboxyl side of lysine residues in Porphyromonas gingivalis was purified from culture supernatant by a combination of ion-exchange chromatography, gel filtration, and affinity chromatography. The molecular mass was 48 kDa and the pI value was 7.3. The enzyme hydrolysed the peptide bonds at the carboxyl side of lysine residues in synthetic substrates and natural proteins.Keywords
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