Influence des facteurs génétique et physiologique chez le Nicotiana sur la néoformation in vitro de fleurs à partir d'assises cellulaires épidermiques et sous-épidermiques

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Abstract
Floral neoformation potentialities in various Nicotiana genotypes having different photoperiodic requirements were analyzed in epidermal and subepidermal cell layers from floral branches cultured in vitro with glucose (30 g/L), indoleacetic acid (IAA, 10− M), and kinetine (Kin, 10−6 M). Direct neoformation of flowers occurred in the long-day interspecific hybrid Nicotiana sylvestris × Nicotiana tomentosiformis, but was not obtained with any of the parent species, suggesting that this phenomenon might depend on interactions between those two genotypes, rather than on euperiod. Results obtained following changes in the medium concerned with auxin and cytokinin (naphtalene acetic acid, 3 × 10−7 M or 5 × 10−7 M; benzyl-adenine, 3 × 10−7 M or 5 × 10−7 M) demonstrated the influence of genotype-environment interaction on de novo differentiation of flowers in Nicotiana rustica. [Translated by the journal]