Transformation and Cotransformation ofGaeumannomyces graministo Phleomycin Resistance
- 1 January 1990
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 80 (10) , 1124-1129
- https://doi.org/10.1094/phyto-80-1124
Abstract
Gaeumannomyces graminis var. graminis and G. g. tritici were transformed to phleomycin resistance by an improved transformation procedure with pAN8-1, a plasmid encoding the ble gene of Streptoalloteichus hindustanus. G. g. graminis was cotransformed with pAN8-1 and pBT3, a plasmid encoding resistant to benomyl. Vector DNA apparently was integrated into the fungal genome in all transformants analyzed, at different sites in the genome and with varying copy numbers. The selected phenotypes (PhleoR or BenR) were stable through mitosis in most transformants. Integrated plasmid DNA was stable through meiosis in all transformants tested.This publication has 17 references indexed in Scilit:
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