Two nuclear DNA binding proteins ofDictyostelium discoideumwith a high affinity for poly(dA)-poly(dT)
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (23) , 8473-8484
- https://doi.org/10.1093/nar/11.23.8473
Abstract
Intergenic regions of the Dictyostelium genome contain an extremely high proportion of AT base pairs. Those intergenic regions which have been subjected to nucleotide sequence analysis are predominantly composed of alternating runs of poly(dA) and poly(dT) and there is evidence to suggest that nucleosomes do not form on such sequences. We have identified two nuclear proteins, of molecular weight 70,000 and 74,000 daltons, which bind only to intergenic regions of a cloned Dictyostelium gene. Binding is specifically inhibited in the presence of synthetic poly(dA) - poly (dT) as competitor. These proteins may play some role in the chromosomal organization of intergenic regions in Dictyostelium discoideum.Keywords
This publication has 23 references indexed in Scilit:
- Specific protein-DNA interaction at four sites flanking the chicken lysozyme geneCell, 1982
- Chicken oviduct progesterone receptor: Location of specific regions of high-affinity binding in cloned DNA fragments of hormone-responsive genesCell, 1982
- T antigen repression of SV40 early transcription from two promotersCell, 1981
- The multiple subunits of discoidin I are encoded by different genesDevelopmental Biology, 1981
- Protein component from drosophila larval nuclei showing sequence specificity for a short region near a major heat-shock protein gene☆Cell, 1981
- Specific interaction of a purified transcription factor with an internal control region of 5S RNA genesCell, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Characterization and transcription analysis of a cloned sequence derived from a major developmentally regulated mRNA of D. discoideumCell, 1979
- Identification and analysis of Dictyostelium actin genes, a family of moderately repeated genesCell, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970