Improved colorimetric determination of serum zinc.
Open Access
- 1 July 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (7) , 1321-1323
- https://doi.org/10.1093/clinchem/23.7.1321
Abstract
We show how zinc may easily be quantified in serum by first using an optimum concentration of guanidine hydrochloride to cause release of zinc from proteins, followed by complexation of released metals with cyanide. The cyanide complex of zinc is preferentially demasked with chloral hydrate, followed by a colorimetric reaction between zinc and 4-(2-pyridylazo)resorcinol. This is a sensitive water-soluble ligand; its complex with zinc has an absorption maximum at 497 nm. Values found by this technique compare favorably with those obtained by atomic absorption spectroscopy.This publication has 2 references indexed in Scilit:
- Simultaneous spectrophotometry of Fe2+ and Cu2+ in serum denatured with guanidine hydrochloride.Clinical Chemistry, 1977
- Study of a Differential Demasking Technic for Serum ZincClinical Chemistry, 1962