Effect of Light on Deamination and Oxidation of Adenylic Compounds in Cotyledons of Pharbitis nil

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Abstract
Since labelling of ureides from adenine‐8‐14C is higher in dark than in light, the influence of light on the deamination and the oxidation of adenylic compounds by cotyledon discs of Pharbitis nit was investigated. Among the three possible adenylic precursors for the deaminative step, adenine was found to be the best compound for the study of the deaminative rate, adenosine being easily hydrolyzed into adenine, and AMP undergoing an apparent complete hydrolysis before entering the cells. By analysis of adenine‐8‐14C metabolism for brief periods, it was determined that the rate of deamination of adenylic compounds was faster in light than in dark. In contrast, the activity of xanthine dehydrogenase was much higher in the dark than in light. The level of the activity of uricase was the same in both light and dark.