GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
Open Access
- 3 April 2000
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 19 (7) , 1494-1504
- https://doi.org/10.1093/emboj/19.7.1494
Abstract
Membrane proteins located on vesicles (v‐SNAREs) and on the target membrane (t‐SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi‐associated ATPase Enhancer of 16 kDa (GATE‐16), that functions as a soluble transport factor. GATE‐16 interacts with N ‐ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v‐SNARE GOS‐28 in an NSF‐dependent manner. We propose that GATE‐16 modulates intra‐Golgi transport through coupling between NSF activity and SNAREs activation.Keywords
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