Determination of Pyruvate Kinase and Creatine Kinase Using Multienzyme Electrodes
- 1 February 1988
- journal article
- analytical biochemistry-and-clinical-analysis
- Published by Taylor & Francis in Analytical Letters
- Vol. 21 (2) , 225-239
- https://doi.org/10.1080/00032718808055748
Abstract
The application of a pyruvate-sensing bienzyme electrode based on lactate dehydrogenase, lactate mono-oxygenase and an oxygen probe for pyruvate kinase (PK) determination in erythrocytes is described. PK can be measured in the range 0.12 – 1.85 μmol/(s1) with good reproducibility and good agreement with the conventional method. Coimmobilization of PK in the enzyme membrane leads to asensor for creatine kinase determination useful in the range 0.1 – 1.76 μmol/(s1).Keywords
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