Precursor cell division during the immune response in vitro: antigen‐induced and “spontaneous” antibody‐forming cells

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Abstract
“Background” or “spontaneous” plaque‐forming cells (PFC) which arise during in vitro culture of mouse spleen cells may be eliminated by a hot thymidine pulse. This does not prevent the subsequent addition of sheep red blood cells (SRBC) or trinitrophenylated (TNP) SRBC eliciting a primary immune response.Using mouse spleen cells, resently primed by carrier (SRBC), it was found that an early “hot pulse” (for the first 24 hours of culture) can eliminate the anti‐SRBC response, but not the primary anti‐TNP response which is dependent on carrier‐specific, θ‐bearing, radioresistant spleen cells.