Testosterone metabolism by pure and mixed cultures of human corynebacteria
Open Access
- 1 March 1987
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 41 (1) , 53-58
- https://doi.org/10.1016/0378-1097(87)90282-5
Abstract
The metabolism of testosterone by 21 different corynebacteria has been analysed in pure and mixed culture. In pure cultures, the occurrence of the following enzymic activities was confirmed; 3α(β)-oxido-reductase, 4-ene-5α(β)-reductase, 17β-oxido-reductase and 17-isomerase. Mixed cultures, formed by co-inoculating the pure strains in groups of 3, showed formation of 3β-hydroxy-5β-androstan-17-one, which was not detected in pure cultures. Mixing cultures which displayed 4-ene-5β-reductase activity (formation of 17β-hydroxy-5β-androstan-3-one(5β-DHT)) with those showing 17-oxido-reductase activity (production of 4-androstene-3,17-dione) sometimes showed evidence for an additive effect, in the formation of 5β-androstane-3,17-dione. Other combinations of strains showed either no effects or inhibitory effects on 5β-androstane-3,17-dione formation. Strain groupings based on the amounts of 5β-DHT or 4-androstene-3,17-dione, as assessed by densitometry of autoradiographs, showed some correlation with previously published groupings based on whole cell protein patterns [1].Keywords
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