Formation of genes coding for hybrid proteins by recombination between related, cloned genes inE. coli
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (16) , 5661-5669
- https://doi.org/10.1093/nar/11.16.5661
Abstract
We describe a method for the formation of hybrid genes by in vivo recombination between two genes with partial sequence homology. DNA structures consisting of plasmid vector sequences, flanked by the α2 interferon gene on the one side and a portion of the al interferon gene (homology about 80%) on the other, were transfected into E.coli SK1592. Appropriate resistance markers allowed the isolation of colonies containing circular plasmids which arose by in vivo recombination between the partly homologous interferon gene sequences. Eleven different recombinant genes were identified, six of which encoded new hybrid inter-ferons not easily accessible by recombinant DNA techniques.Keywords
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