High Performance Liquid Chromatographic Determination of Neomycin in Milk Using a Hisep Column
- 1 July 1990
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 13 (12) , 2475-2487
- https://doi.org/10.1080/01483919008049047
Abstract
A high performance liquid chromatographic (HPLC) method for the determination of neomycin in milk is described. Milk is passed directly through an amberlite CG-50 ion exchange resin column, and the neomycin which is retained on the column is derivatized with ortho-phthalaldehyde (OPA) reagent. The derivatized neomycin is eluted from the column with potassium borate buffer/methanol and analyzed by HPLC. A HISEP HPLC column with fluororoetric detection was used. Recoveries ranged from 94 to 102% in samples fortified between 0.1 to 5ppra levels. The detection limit is 50ppb.This publication has 7 references indexed in Scilit:
- High-Performance Liquid Chromatography for Analyses of Antibiotics in Biological FluidsJournal of Liquid Chromatography, 1983
- Determination of Neomycin Residues in Eggs and Stability of Residues After CookingJournal of AOAC INTERNATIONAL, 1978
- Improved microbiological assay procedures for dihydrostreptomycin residues in milk and dairy productsApplied and Environmental Microbiology, 1978
- Comparative Study of Responses to Neomycins B and C by Microbiological and Gas-Liquid Chromatographic Assay MethodsApplied Microbiology, 1969
- A CLINICAL APPRAISAL OF NEOMYCINAnnals of Internal Medicine, 1950
- ANTIMICROBIAL PROPERTIES OF NEOMYCIN1950