Toward a steady‐state pore limit electrophoresis dimension for native proteins in two‐dimensional polyacrylamide gel electrophoresis
- 1 January 1989
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 10 (3) , 182-185
- https://doi.org/10.1002/elps.1150100305
Abstract
Polyacrylamide gel electrophoresis in linear pore gradients (4.8 to 48 %T, 5 %CBis) provides for migration arrest, in a practical sense, after about 5000 Vh for proteins of 290 and 450 kDa, but not for smaller proteins over 20 000 Vh. The arrest is not due to inadequate field strength nor is it caused by water redistribution within pore gradien gels. The possibility is being discussed that exponential pore gradients, and a higher or a lower degree of crosslinking suggested by the literature may be remedies for the present failure to arrest the migration of smaller proteins.This publication has 13 references indexed in Scilit:
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