Dipetalonema viteae (Nematoda: Filarioidea): Culture of Third-Stage Larvae to Young Adults in Vitro
- 7 July 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 221 (4606) , 161-163
- https://doi.org/10.1126/science.6682998
Abstract
Infective third-stage larvae of Dipetalonema viteae (Nematoda: Filarioidea) were cultured to young adults in a cell-free culture system. Third-stage larvae from the tick vector grew, developed, and molted twice in a medium containing NCTC 135 and Iscove's modified Dulbecco's medium supplemented with fetal bovine serum under a gas phase of 95 percent nitrogen and 5 percent carbon dioxide. The availability of such a culture system for filariids should facilitate studies of their immunology, biochemistry, and sensitivity to drugs.This publication has 9 references indexed in Scilit:
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