Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3' ends in vivo
- 2 January 2004
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 32 (1) , 255-262
- https://doi.org/10.1093/nar/gkh182
Abstract
Although correct tRNA 3¢ ends are crucial for protein biosynthesis, generation of mature tRNA 3¢ ends in eukaryotes is poorly understood and has so far only been investigated in vitro. We report here for the first time that eukaryotic tRNA 3¢ end matur- ation is catalysed by the endonuclease RNase Z in vivo. Silencing of the JhI-1 gene (RNase Z homo- log) in vivo with RNAi in Drosophila S2 cultured cells causes accumulation of nuclear and mitochon- drial pre-tRNAs, suggesting that JhI-1 encodes both forms of the tRNA 3¢ endonuclease RNase Z, and establishing its biological role in endonucleolytic tRNA 3¢ end processing. In addition our data show that in vivo 5¢ processing of nuclear and mitochon- drial pre-tRNAs occurs before 3¢ processing.Keywords
This publication has 41 references indexed in Scilit:
- Animal mitochondrial genomesNucleic Acids Research, 1999
- The 3′ end CCA of mature tRNA is an antideterminant for eukaryotic 3′-tRNaseRNA, 1999
- RIBONUCLEASE P: Unity and Diversity in a tRNA Processing RibozymeAnnual Review of Biochemistry, 1998
- 5′ end maturation and RNA editing have to precede tRNA 3′ processing in plant mitochondriaProceedings of the National Academy of Sciences, 1998
- Two birds with one stone: genes that encode products targeted to two or more compartmentsPlant Molecular Biology, 1998
- The Yeast La Protein Is Required for the 3′ Endonucleolytic Cleavage That Matures tRNA PrecursorsCell, 1997
- Purification and Characterization of the Precursor tRNA 3′-End Processing Nuclease fromAspergillus nidulansBiochemical and Biophysical Research Communications, 1997
- NUCLEOLYTIC PROCESSING OF A TRANSFER RNAARG-TRANSFER RNAASP DIMERIC PRECURSOR BY A HOMOLOGOUS COMPONENT FROM SACCHAROMYCES-CEREVISIAE1985
- PURIFICATION AND CHARACTERIZATION OF AN ENDONUCLEASE FROM XENOPUS-LAEVIS OVARIES WHICH ACCURATELY PROCESSES THE 3' TERMINUS OF HUMAN PRE-TRANSFER RNA(I)MET (3' PRE-TRANSFER RNASE)1985
- Processing of precursor tRNAs in Drosophila. Processing of the 3‘ end involves an endonucleolytic cleavage and occurs after 5‘ end maturation.Journal of Biological Chemistry, 1985