Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC
- 22 June 1987
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 218 (1) , 167-172
- https://doi.org/10.1016/0014-5793(87)81040-2
Abstract
Up to 80% of the calcium-binding protein BM-40 could be extracted from a tumor basement membrane with a physiological buffer containing 10 mM EDTA. About half of its amino acid sequence was determined by Edman degradation demonstrating identity with cDNA deduced sequences of bone osteonectin and SPARC.Keywords
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