A Colorimetric Method for Estimating Acetoacetate

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Abstract
The use of sodium nitroprusside in alkaline solution for determination of acetoacetate was made quantitative by addition of aminoacetic acid which served to intensify and stabilize the color produced. Optimal conditions for the reaction were determined and a study of interfering substances that may be present in biological fluids (sulfhydryl compounds, pyruvate, acetone, and creatinine) was made. Creatinine was found to be the only substance that gave significant interference at physiological levels. A validation procedure was described and methods for blood plasma and urine were suggested.

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