Determination of Prekallikrein in Plasma by Means of a Chromogenic Tripeptide Substrate for Plasma Kallikrein
- 1 January 1979
- book chapter
- Published by Springer Nature
Abstract
A method for plasma prekallikrein determination utilizing a chromogenic tripeptic substrate is presented. The method has a good reproducibility and can easily be automized. Several parameters have been optimized. By using mixtures of deficient plasmas and pooled normal plasma or purified factors it was proved that prekallikrein was the factor determined and that more than 10% (of normal plasma concentration) of FXII and HMW kininogen were essential for the activation of prekallikrein in our method. Further experiments showed that the method was fairly selective and was not influenced by inhibitors present in normal plasma. The later finding was attributed to the high dilution of plasma made possible by using a potent activator and a sensitive substrate.Keywords
This publication has 11 references indexed in Scilit:
- Role of surface in surface-dependent activation of Hageman factor (blood coagulation Factor XII)Proceedings of the National Academy of Sciences, 1978
- The purification of a human plasma kallikrein with weak plasminogen activator activityThrombosis Research, 1978
- A new sensitive and highly specific chromogenic peptide substrate for Factor XaThrombosis Research, 1977
- Role of high-molecular-weight kininogen in surface-binding and activation of coagulation Factor XI and prekallikrein.Proceedings of the National Academy of Sciences, 1977
- REQUIREMENTS FOR CONTACT ACTIVATION OF HUMAN HAGEMAN FACTORAnnals of the New York Academy of Sciences, 1977
- The Hageman Factor Dependent Pathways of Coagulation, Fibrinolysis, and Kinin-GenerationSeminars in Thrombosis and Hemostasis, 1976
- Plasma Inhibitors of The Hageman Factor Dependent PathwaysSeminars in Thrombosis and Hemostasis, 1976
- The inhibition of human plasma Kallikrein by antithrombin IIIThrombosis Research, 1975