Integration of human immunodeficiency virus type 1 DNA in vitro.
Open Access
- 1 June 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (11) , 4164-4168
- https://doi.org/10.1073/pnas.87.11.4164
Abstract
A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.This publication has 7 references indexed in Scilit:
- Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractionsPublished by Elsevier ,2004
- Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expressionJournal of Virology, 1989
- A nucleoprotein complex mediates the integration of retroviral DNA.Genes & Development, 1989
- Retroviral integration: structure of the initial covalent product and its precursor, and a role for the viral IN protein.Proceedings of the National Academy of Sciences, 1989
- Retroviral DNA integration: Structure of an integration intermediateCell, 1988
- Correct integration of retroviral DNA in vitroCell, 1987