Nature of the Binding Site Around and Reactivity of Histidine-15 in Lysozyme
- 1 February 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 95 (2) , 503-510
- https://doi.org/10.1093/oxfordjournals.jbchem.a134632
Abstract
Bromoacetamide derivatives having n -alkyl substituents (BrCH 2 CONH(CH 2 ) n H, 1- n ) and carboxyalkyl substituents (BrCH 2 CONH(CH 2 ) n COOH, 2- n ) react with His-15 in lysozyme exclusively at N 2 at pH 5.5 and 40°C. Kinetic studies suggest that lysozyme has a small hydrophobic pocket that binds these reagents in the vicinity of His-15.Keywords
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