Dissociation of suction blister roof epidermis with trypsin and desoxyribonuclease into viable single cells
Open Access
- 1 January 1981
- journal article
- research article
- Published by Medical Journals Sweden AB in Acta Dermato-Venereologica
- Vol. 61 (1) , 58-61
- https://doi.org/10.2340/00015555615861
Abstract
Human epidermis, obtained in vivo by the suction blister method, was dissociated with trypsin and desoxyribonuclease into a single-cell suspension. Autoradiographic analysis of the blister roof epidermis and of the epidermal cell suspension was performed to show that neither the suction procedure nor the enzymatic dissociation affected DNA synthesis of the epidermal cells.This publication has 4 references indexed in Scilit:
- LANGERHANS CELL - ITS ORIGIN, NATURE, AND FUNCTION1978
- SELECTIVE SEPARATION OF EPIDERMAL CELLS FOR CYTOFLUOROMETRIC ESTIMATION OF NUCLEAR DNAACTA HISTOCHEMICA ET CYTOCHEMICA, 1978
- Preparation and Characterization of a Viable Suspension of Postembryonic Human Epidermal **From the Division of Dermatology, Department of Medicine, University of North Carolina Medical School, Chapel Hill, North Carolina.Journal of Investigative Dermatology, 1967
- “ECM”: Its nature, origin and function in cell aggregationExperimental Cell Research, 1963