Ultrastructure and phylogenetic analysis of ‘Candidatus Neoehrlichia mikurensis' in the family Anaplasmataceae, isolated from wild rats and found in Ixodes ovatus ticks
Top Cited Papers
- 1 September 2004
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 54 (5) , 1837-1843
- https://doi.org/10.1099/ijs.0.63260-0
Abstract
A novel bacterium that infects laboratory rats was isolated from wild Rattus norvegicus rats in Japan. Transmission electron microscopy of the spleen tissue revealed small cocci surrounded by an inner membrane and a thin, rippled outer membrane in a membrane-bound inclusion within the cytoplasm of endothelial cells. Phylogenetic analysis of the 16S rRNA gene sequence of the bacterium found in R. norvegicus rats and Ixodes ovatus ticks in Japan revealed that the organism represents a novel clade in the family Anaplasmataceae, which includes the Schotti variant found in Ixodes ricinus ticks in the Netherlands and the Ehrlichia-like Rattus strain found in R. norvegicus rats from China. The novel clade was confirmed by phylogenetic analysis of groESL sequences found in R. norvegicus rats and Ixodes ovatus ticks in Japan. No serological cross-reactivity was detected between this bacterium and members of the genera Anaplasma, Ehrlichia or Neorickettsia in the family Anaplasmataceae. It is proposed that this new cluster of bacteria should be designated ‘Candidatus Neoehrlichia mikurensis’.Keywords
This publication has 25 references indexed in Scilit:
- Molecular Characterization of Aegyptianella pullorum ( Rickettsiales , Anaplasmataceae )Journal of Clinical Microbiology, 2003
- Ehrlichia‐like Organism Gene Found in Small Mammals in the Suburban District of Guangzhou of ChinaAnnals of the New York Academy of Sciences, 2003
- Candidatus Ehrlichia WalkeriiAnnals of the New York Academy of Sciences, 2003
- Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila.International Journal of Systematic and Evolutionary Microbiology, 2001
- Persistent infection in Neotoma fuscipes (Muridae: Sigmodontinae) with Ehrlichia phagocytophila sensu lato.The American Journal of Tropical Medicine and Hygiene, 2001
- Identification ofEhrlichiaspp. andBorrelia burgdorferiinIxodesTicks in the Baltic Regions of RussiaJournal of Clinical Microbiology, 2001
- Ehrlichial Proliferation and Acute Hepatocellular Necrosis in Immunocompetent Mice Experimentally Infected with the HF Strain of Ehrlichia, Closely Related to Ehrlichia chaffeensisJournal of Comparative Pathology, 2001
- Ultrastructural and Antigenic Characterization of a Granulocytic Ehrlichiosis Agent Directly Isolated and Stably Cultivated from a Patient in New York StateThe Journal of Infectious Diseases, 1997
- Taxonomic Note: Implementation of the Provisional Status Candidatus for Incompletely Described ProcaryotesInternational Journal of Systematic and Evolutionary Microbiology, 1995
- The tribe Ehrlichieae and ehrlichial diseasesClinical Microbiology Reviews, 1991